Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis.
Identifieur interne : 000696 ( Main/Exploration ); précédent : 000695; suivant : 000697Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis.
Auteurs : Dayong Ding [République populaire de Chine] ; Pengyun Li [République populaire de Chine] ; Xueming Zhang [République populaire de Chine] ; Shri Ramaswamy [États-Unis] ; Feng Xu [République populaire de Chine]Source :
- Bioresource technology [ 1873-2976 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- effets des médicaments et des substances chimiques : Populus.
- métabolisme : Lignine, Polyosides, Populus.
- pharmacologie : Sérumalbumine bovine.
- Adsorption, Biomasse, Hydrolyse.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Lignin, Polysaccharides.
- drug effects : Populus.
- metabolism : Populus.
- chemical , pharmacology : Serum Albumin, Bovine.
- Adsorption, Biomass, Hydrolysis.
Abstract
A cost efficient synergistic strategy combining mild alkaline pretreatment (0.5-5% NaOH at 70 °C for 60 min) and bovine serum albumin (BSA) blocking of lignin was evaluated for effective conversion of poplar. The highest glucose yield of 69.2% was obtained for 5% alkaline pretreated sample, which was 4.4 times that of untreated sample. The enhanced enzymatic hydrolysis was attributed to significant hemicelluloses removal with limited delignification. Delignification mainly occurred in secondary wall, leading to more open cell wall structure, thus facilitating better transport of enzyme. Hemicelluloses removal helped split adjacent microfibrils, thus increased the specific sites for cellulase binding. After BSA addition in enzymatic hydrolysis, cellulose conversion further improved to 78.4% with 33% reduction of cellulase dosage due to decreased non-specific adsorption of cellulase on residual lignin. The utilization of synergistic alkaline pretreatment - BSA strategy may improve the overall economics of biomass conversion and successful commercial implementation of biorefineries.
DOI: 10.1016/j.biortech.2018.11.024
PubMed: 30447624
Affiliations:
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Electronic address: xfx315@bjfu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption (MeSH)</term><term>Biomass (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Lignin (metabolism)</term><term>Polysaccharides (metabolism)</term><term>Populus (drug effects)</term><term>Populus (metabolism)</term><term>Serum Albumin, Bovine (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adsorption (MeSH)</term><term>Biomasse (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Lignine (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (métabolisme)</term><term>Sérumalbumine bovine (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Polyosides</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sérumalbumine bovine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adsorption</term><term>Biomass</term><term>Hydrolysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adsorption</term><term>Biomasse</term><term>Hydrolyse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cost efficient synergistic strategy combining mild alkaline pretreatment (0.5-5% NaOH at 70 °C for 60 min) and bovine serum albumin (BSA) blocking of lignin was evaluated for effective conversion of poplar. The highest glucose yield of 69.2% was obtained for 5% alkaline pretreated sample, which was 4.4 times that of untreated sample. The enhanced enzymatic hydrolysis was attributed to significant hemicelluloses removal with limited delignification. Delignification mainly occurred in secondary wall, leading to more open cell wall structure, thus facilitating better transport of enzyme. Hemicelluloses removal helped split adjacent microfibrils, thus increased the specific sites for cellulase binding. After BSA addition in enzymatic hydrolysis, cellulose conversion further improved to 78.4% with 33% reduction of cellulase dosage due to decreased non-specific adsorption of cellulase on residual lignin. The utilization of synergistic alkaline pretreatment - BSA strategy may improve the overall economics of biomass conversion and successful commercial implementation of biorefineries.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30447624</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>273</Volume><PubDate><Year>2019</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis.</ArticleTitle><Pagination><MedlinePgn>231-236</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(18)31544-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2018.11.024</ELocationID><Abstract><AbstractText>A cost efficient synergistic strategy combining mild alkaline pretreatment (0.5-5% NaOH at 70 °C for 60 min) and bovine serum albumin (BSA) blocking of lignin was evaluated for effective conversion of poplar. The highest glucose yield of 69.2% was obtained for 5% alkaline pretreated sample, which was 4.4 times that of untreated sample. The enhanced enzymatic hydrolysis was attributed to significant hemicelluloses removal with limited delignification. Delignification mainly occurred in secondary wall, leading to more open cell wall structure, thus facilitating better transport of enzyme. Hemicelluloses removal helped split adjacent microfibrils, thus increased the specific sites for cellulase binding. After BSA addition in enzymatic hydrolysis, cellulose conversion further improved to 78.4% with 33% reduction of cellulase dosage due to decreased non-specific adsorption of cellulase on residual lignin. The utilization of synergistic alkaline pretreatment - BSA strategy may improve the overall economics of biomass conversion and successful commercial implementation of biorefineries.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Dayong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Pengyun</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xueming</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ramaswamy</LastName><ForeName>Shri</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China. Electronic address: xfx315@bjfu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>27432CM55Q</RegistryNumber><NameOfSubstance UI="D012710">Serum Albumin, Bovine</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012710" MajorTopicYN="N">Serum Albumin, Bovine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">BSA</Keyword><Keyword MajorTopicYN="N">Enzymatic hydrolysis</Keyword><Keyword MajorTopicYN="N">Hemicelluloses removal</Keyword><Keyword MajorTopicYN="N">Poplar cell wall</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30447624</ArticleId><ArticleId IdType="pii">S0960-8524(18)31544-X</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2018.11.024</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Minnesota</li></region><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ding, Dayong" sort="Ding, Dayong" uniqKey="Ding D" first="Dayong" last="Ding">Dayong Ding</name></noRegion><name sortKey="Li, Pengyun" sort="Li, Pengyun" uniqKey="Li P" first="Pengyun" last="Li">Pengyun Li</name><name sortKey="Xu, Feng" sort="Xu, Feng" uniqKey="Xu F" first="Feng" last="Xu">Feng Xu</name><name sortKey="Zhang, Xueming" sort="Zhang, Xueming" uniqKey="Zhang X" first="Xueming" last="Zhang">Xueming Zhang</name></country><country name="États-Unis"><region name="Minnesota"><name sortKey="Ramaswamy, Shri" sort="Ramaswamy, Shri" uniqKey="Ramaswamy S" first="Shri" last="Ramaswamy">Shri Ramaswamy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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